Research Paper Volume 12, Issue 7 pp 5625—5639

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p

Figure 5. LncRNA 2310043L19Rik induces G0/G1 to S transition and cell proliferation. (A) Proliferating C2C12 cells were labeled with EdU after transfection with 2310043L19Rik overexpression vector (lnc-231), control vector (pcDNA3.1) according to the manufacturer’s instructions. The cell nuclei were stained with DAPI. The percentage of EdU+ cells was quantified (B). (C) Proliferating C2C12 cells were labeled with EdU after transfection with 2310043L19Rik inhibitor (silnc-231), or siNC. The cell nuclei were stained with DAPI. The percentage of EdU+ cells was quantified (D). (E) Real-time PCR analysis of Ki67 and cell cycle-dependent kinases (CDK2, CDK4, CDK6) mRNA expression in C2C12 transfected with control pcDNA3.1 or lnc-231 then cultured in GM for 24 h. (F) Real-time PCR analysis of Ki67 and cell cycle-dependent kinases (CDK2, CDK4, CDK6) expression in C2C12 transfected with siNC or silnc-231 cultured in GM for 24 h. Western blot results analysis of 2310043L19Rik overexpression (G) and knockdown (H) on the protein expression levels of CDK2 and CDK6 in C2C12 cells cultured in GM for 48 h.