Research Paper Volume 12, Issue 7 pp 5625—5639

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p

Figure 8. lncRNA 2310043L19Rik promotes proliferation of myoblast cells by attenuating function of miR-125a-5p. (A) Proliferating C2C12 cells were labeled with EdU after transfection with control NC (pcDNA3.1+ mimics NC), mimics miR-125a-5p, mimics miR-125a-5p+lnc-231, lnc-231. The cell nuclei were stained with DAPI. The percentage of EdU+ cells was quantified (B). (C) Flow cytometry analysis of the percentage of S-phase cells after transfection with control NC (pcDNA3.1+ mimics NC), mimics miR-125a-5p, mimics miR-125a-5p+lnc-231, lnc-231. Statistical analysis of S-phase cells ratio (D). (E) The determination of cell viability using the CCK-8 assay. Mean values ± SEM, n=3, *P< 0.05, **P< 0.01.