Research Paper Volume 12, Issue 7 pp 5640—5650

Inhibition of miR-19a partially reversed the resistance of colorectal cancer to oxaliplatin via PTEN/PI3K/AKT pathway

Figure 2. Effect of miR-19a on regulating the oxaliplatin sensitivity of CRC cells. (A) QRT-PCR analysis was performed to detect the expression of miR-19a in SW480, SW480/R, HT29 and HT29/R. *P<0.05. (B) Transfection with miR-19a mimics (50 pmol/ml) increased the cellular level of miR-19a in SW480 and HT29 cells. *P<0.05. (C) Transfection with miR-19a mimics (50 pmol/ml) decreased the sensitivity of SW480 and HT29 cells to oxaliplatin (10 μM) treatment. *P<0.05. (D) Transfection with anti-miR-19a (50 pmol/ml) decreased the cellular level of miR-19a in SW480/R and HT29/R cells. *P<0.05. (E) Transfection with anti-miR-19a (50 pmol/ml) increased the sensitivity of SW480/R and HT29/R cells to oxaliplatin (10 μM) treatment. *P<0.05.