Research Paper Volume 12, Issue 7 pp 5675—5692

STAT5A induced LINC01198 promotes proliferation of glioma cells through stabilizing DGCR8

Figure 1. Up-regulated LINC01198 was linked with poor overall prognosis in glioma. (A) heterogeneous expression of LINC01198 in glioma as well as normal brain tissue, with LINC01198 level being markedly varied from strong positive, denoting as +++, to weak positive, denoting as + among different glioma cases, as evidenced by ISH staining. Scale bar, 50μm; scale bar in inset, 5μm. (B) the enumeration of LINC01198 expression evaluated by ISH in glioma and its paired normal controls, totaling 100. (C) qRT-PCR detection of LINC01198 in another independent cohort comprising 70 cases of fresh frozen glioma tissues and its matched normal controls. Two tailed, paired T-test was used to analyze the significant difference (t=8.220, df=69, P<0.0001). (D) Overall survival analysis of LINC01198 by Kaplan-Meier survival curve in glioma, totaling 100. Of which, the number of cases detected with high expression of LINC01198 by ISH was 68; the remainder was 32 with low expression of LINC01198. Log-Rank Chi-square was used to analyze the difference of survival (χ2=10.06, P=0.0015). (E) Likewise and incidentally, disease-free survival was also analyzed by Log-Rank Chi-square was used to analyze the difference of survival (χ2=8.381, P=0.0038). (F) Similarly, Overall survival analysis was performed of LINC01198 in 70 cases of fresh frozen glioma tissues, totaling 70 cases. Log-Rank Chi-square was used to analyze the difference of survival (χ2=6.751, P=0.0094). (G) Overall survival analysis of LINC01198 expression data in patients diagnosed with low grade glioma (LGG) derived from TCGA database.