Research Paper Volume 12, Issue 8 pp 6570—6585

Long non-coding RNA MALAT1 exacerbates acute respiratory distress syndrome by upregulating ICAM-1 expression via microRNA-150-5p downregulation

Figure 4. Downregulated ICAM-1 results in inhibited HPMEC apoptosis and decreased expression of pro-inflammatory cytokines and adhesion factors. (A) Expression of ICAM-1 in HPMECs. (B) Expression of pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in HPMECs transfected with sh-ICAM-1, as determined by ELISA. (C) The expression of endothelial cell adhesion molecules (E-selectin and ICAM-1) in HPMECs transfected with sh-ICAM-1 detected by immunofluorescence (× 200). (D) HPMEC apoptosis following transfection with sh-ICAM-1 detected by TUNEL assay (× 200). (E) Expression of Bcl-2, Bax, cleaved caspase 3 and Caspase3 in HPMECs transfected with sh-ICAM-1 detected by Western blot analysis. (F) The expression of pro-inflammatory cytokines (IL-6, IL-1β and TNF-α) in HPMECs determined by ELISA. (G) The expression of endothelial cell adhesion molecules (E-selectin and ICAM-1), as detected by immunofluorescence (× 200). (H) HPMEC apoptosis detected by TUNEL assay (× 200). (I) The expression of Bcl-2, Bax, cleaved caspase 3 and Caspase3 in HPMECs detected by Western blot analysis. * p < 0.05 vs. cells transfected with oe-ICAM-1 NC, sh-ICAM-1 NC or miR-150-5p mimic + oe-ICAM-1 NC. The data were measurement data and expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test and those among multiple groups were analyzed by one-way ANOVA, with Tukey's post hoc test. The cell experiment was repeated three times independently.