Research Paper Volume 12, Issue 7 pp 5693—5715

Indian Hedgehog regulates senescence in bone marrow-derived mesenchymal stem cell through modulation of ROS/mTOR/4EBP1, p70S6K1/2 pathway

Figure 1. IHH signaling pathway downregulated in aged BMSC. (A) Morphology of non-senescent and senescent BMSC. (B) Non-senescent and senescent BMSC stained by SA-β-gal stain. (C) BMSC (n = 4) were incubated with or without senescence-induction medium for 8 days. IHH, PTCH1/2, SA-β-gal, p53, p16, ALP-BMA, FABP4, and LPL genes expressions were measured by RT-PCR. GAPDH was used as a housekeeping gene. (D) Isolated BMSC (n = 4) were incubated with or without senescence-induction medium for 8 days. IHH protein expression was measured by Western Blot. β-actin was used as an internal control. (E) Isolated BMSC from young and old donors (n = 4) were analyzed for IHH gene expression by RT-PCR. GAPDH was used as a housekeeping gene. Results presented as mean ± SEM. *P <0.05, **P < 0.01.