Research Paper Volume 12, Issue 7 pp 5693—5715

Indian Hedgehog regulates senescence in bone marrow-derived mesenchymal stem cell through modulation of ROS/mTOR/4EBP1, p70S6K1/2 pathway

Figure 10. IHH inhibited phosphorylation of 4EBP1 and p70S6K1/2 in BMSC. (A) BMSC (n = 5) were transfected with siRNA negative control, siRNA IHH, rIHH, INK128, or rapamycin for 48hours. 4EBP1 and p70S6K1/2 total and phosphorylated proteins expressions were measured by Western Blot. β-actin was used as an internal control. (B) siRNA IHH-transfected BMSC (n=5) were incubated with or without rapamycin for 48hours. P16 and P53 proteins expressions were measured by Western Blot. β-actin was used as an internal control. All results were normally distributed and shown as mean ± SEM. *P <0.05, ****P<0.0001.