Research Paper Volume 12, Issue 7 pp 5693—5715

Indian Hedgehog regulates senescence in bone marrow-derived mesenchymal stem cell through modulation of ROS/mTOR/4EBP1, p70S6K1/2 pathway

Figure 2. IHH knockdown induced BMSC senescence. (A) BMSC transfected with NC siRNA and IHH siRNA stained by SA-β-gal stain. (B) BMSC (n = 5) were transfected with siRNA negative control or siRNA IHH for 24hours. TP53, CDKN2A, SA-β-gal, mTOR, PIK3, GDF11, COX-2, IDO1, IL-6, HGF, and TGFβ genes expressions were measured by RT-PCR. GAPDH was used as a housekeeping gene. (C) BMSC (n = 5) were transfected with siRNA negative control or siRNA IHH for 48hours. P53, P16, PI3K, and p-PI3K proteins expressions were measured by Western Blot. β-actin was used as an internal control. Results presented as mean ± SEM. *P <0.05, **P < 0.01, ***P < 0.001, ****P.