Figure 6. IHH reversed aging-related genes and promoted genes of proper differentiation. (A) BMSC (n = 5) were incubated with and without IHH for 24hours. Aging-related genes, TP53, CDKN2A, SA-β-gal, and mTOR, adipogenesis markers, PPARγ and FABP4, and osteogenesis markers, ALP-BMA, and osteocalcin genes expressions were measured by RT-PCR. GAPDH was used as a housekeeping gene. (B) BMSC from SAMP8 mice (n = 4) were incubated with and without IHH for 24hours. Aging-related genes, p16 and TP53 were measured by RT-PCR. β-actin was used as a housekeeping gene. All data obtained are indicated as mean ± SEM. *P <0.05, **P < 0.01, ***p <0.001.