Research Paper Volume 12, Issue 7 pp 5812—5831

Klotho antagonizes pulmonary fibrosis through suppressing pulmonary fibroblasts activation, migration, and extracellular matrix production: a therapeutic implication for idiopathic pulmonary fibrosis

Figure 3. Kl expression decreases in the murine pulmonary fibrosis model induced by bleomycin. Protein levels of fibronectin, KL, and α-Tubulin (A) were assessed by western blotting and mRNA levels of Kl were measured by qPCR (B) in the total lung lysates from mice 14, 21, and 28 days after intratracheally administering a single dose of PBS (Ctrl, white bars) or bleomycin (BLM, black bars). mRNA levels of Kl in the primary pulmonary fibroblasts (C) isolated from mice 14, 21, and 28 days after intratracheally administering a single dose of PBS (Ctrl, white bars) or bleomycin (BLM, black bars) were analyzed by qPCR. *P < 0.05 or **P < 0.01 vs. Ctrl. 5-7 animals per group.