Research Paper Volume 12, Issue 8 pp 6600—6610

PHTF2 regulates lipids metabolism in gastric cancer

Figure 3. Effects of PHTF2 on the intracellular contents of lipids and key lipid metabolic enzymes in gastric cancer cells. (A) Identification of gene sets enriched in phenotypes correlated with PHTF2 by GSEA using TCGA data. (B) Heat map of core enrichment genes in the gene set KEGG FATTY ACID METABOLISM. (C) Up: Western blot showed PHTF2 protein expression in indicated cells. Down: RT-qPCR analysis for mRNA levels of the key lipid metabolic enzymes ACSL2, ACSL3, HADH, ADH4 and ALDH1B in the indicated cells. (D, E) Cellular content of triglycerides (D) and phospholipids (E) was detected in the indicated cells. (F) The neutral lipids content was detected by double staining with BODIPY 493/503 dye in the indicated cells. Scale bars (10 μm). (G, H) Cellular content of triglycerides (G) and phospholipids (H) was detected in the indicated cells. (I) The neutral lipids content was detected by double staining with BODIPY 493/503 dye in the indicated cells. Scale bars (10 μm). (J) RT-qPCR analysis for ACSL1 mRNA levels in the indicated cells.