Figure 4. HO-1 upregulates Gas6 expression in RPMI8226 cells. (A, B) mRNA expression of HO-1 and Gas6 in RPMI8226 cells were measured by qRT-PCR. (C-E) Western blot and semi-quantitative analysis of HO-1 and Gas6 protein levels in RPMI8226 cells-treated with hemin for 24 h. (F) Gas6 protein in culture supernatants from RPMI8226 cells were measured by Gas6 ELISA. (G, H) HO-1 and Gas6 mRNA levels in RPMI8226 cells were measured by qRT-PCR after transfection with empty vector (EV) and HO-1 recombinant lentiviral. (I–K) The effects of HO-1 overexpression on Gas6 protein expression level was shown in RPMI8226 cells. (L) The effects of HO-1 overexpression on Gas6 secretion in culture supernatants from RPMI8226 cells. Data are expressed as mean ± SD (n = 4). *P < 0.05 vs. RPMI8226 group; #P < 0.05 vs. RPMI8226-EV group.