Research Paper Volume 12, Issue 8 pp 6611—6629

Heme oxygenase-1 inhibition mediates Gas6 to enhance bortezomib-sensitivity in multiple myeloma via ERK/STAT3 axis

Figure 7. Combination with HO-1 inhibition enhances bortezomib-induced apoptosis via suppressing Gas6 production. (AC) Caspase-3 and Bcl-2 relative protein levels were detected by western blot analysis. n=3, *P<0.05. (D) Cell apoptosis (Q2+Q3) of U266 cells assessed by flow cytometry after transfection with HO-1 siRNA and treatment with or without exogenous Gas6 or Gas6 neutralizing antibody for 24 h. n=4, *P<0.05. (E) Flow cytometry was used to assess human CD138+ cells apoptosis after treatment with HO-1 inhibitor ZnPPIX with or without exogenous Gas6 or Gas6 neutralizing antibody for 24 h. n=3, *P<0.05.