Figure 2. Genistein induces apoptosis in EsC cells and arrests cell cycle in G0/G1 phase. The cell apoptosis of (A, B) Eca-109, (C, D) EC9706 and (E, F) CaES-17 cells treated with various concentrations of genistein for 24 h was detected using flow cytometry. The cell cycle of (G, H) Eca-109, (I, J) EC9706 and (K, L) CaES-17 cells treated with different concentrations of genistein for 24 h was detected using flow cytometry. All experiments were independently repeated in triplicate. Data are analyzed using one-way ANOVA with Dunnett’s test and presented as the mean ± SD. *P<0.05.