Research Paper Volume 12, Issue 8 pp 6667—6679

Ultraconserved element uc.333 increases insulin sensitivity by binding to miR-223

Figure 1. The level of uc.333 was reduced in the livers of the HFD-fed mice and db/db mice. (A) Expression of ucRNAs in the liver of db/db mice analyzed using real-time PCR (n = 6). (B) Expression of ucRNAs in the liver of HFD-fed mice analyzed using real-time PCR (n = 6). (C) Expression of uc.333 in liver of 10-week-old mice injected with IL-6 16 μg /mL and TNF-α 16 μg /mL for 7 days. (D) Expression of uc.333 in HepG2 cells induced with 20 nM TNF-α and 20 nM IL-6. Data are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group (Student’s t test).