Research Paper Volume 12, Issue 8 pp 6667—6679

Ultraconserved element uc.333 increases insulin sensitivity by binding to miR-223

Figure 3. Overexpression of uc.333 ameliorated IR. (A) Immunofluorescence assay showed that Ad-NC or Ad-uc.333 transfected into HepG2 cells with equal transfection efficiency.(B) Level of uc.333 in the HepG2 cells transfected with Ad-uc.333 or Ad-NC for 48 h. (C) Glycogen content in HepG2 cells after transfection with Ad-uc.333 or Ad-NC for 48 h. (D) Expression of glucose in HepG2 cells transfected with Ad-uc.333m or Ad-NC for 48 h. (E) Protein levels of P-AKT, AKT, P-GSK, GSK, and GAPDH in HepG2 cells transfected with Ad- uc.3333 or Ad-NC for 48 h, as analyzed using Western blot. (F) The mRNA levels of G6Pase, PGC-1α and PEPKC were also decreased after overexpression of uc.333 compared with that of Ad-NC Data are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group (Student’s t test).