Research Paper Volume 12, Issue 8 pp 6667—6679

Ultraconserved element uc.333 increases insulin sensitivity by binding to miR-223

Figure 4. Inhibition of uc.333-promoted IR. (A) The results of quantitative RT-PCR analysis for the expression of uc.333 in HepG2 cells transfected with negative control (NC) or siRN. (B) Determination of glycogen content in HepG2 cells after transfection with si-uc.333 or NC for 48 h. (C) Expression of glucose in HepG2 cells transfected with si-uc.333m or NC for 48 h. (D) Expression of P-AKT, AKT, P-GSK, GSK, and GAPDH in HepG2 cells transfected with si-uc.333 or NC for 48 h, as analyzed using Western blot. Data are mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group (Student’s t test).