Research Paper Volume 12, Issue 8 pp 6680—6699

Long intergenic non-protein coding RNA 324 prevents breast cancer progression by modulating miR-10b-5p

Figure 6. LINC00324 restored E-cadherin expression by sponging miR-10b-5p. (A) Binding site of miR-10b-5p on E-cadherin. (B) Kaplan–Meier analysis for overall survival based on low and high E-cadherin levels (the KM Plotter database). (C) Relative expression of CDH1 in MDA-MB-231 cells transfected with miR-10b-5p mimics or inhibitor. (D) Western blot analysis of E-cadherin protein expression in MDA-MB-231 cells after transfection with miR-10b-5p mimics or inhibitor. (E) Luciferase assay of miR-10b-5p overexpression MDA-MB-231 cells transfected with pmirGLO-3′ UTR reporter of E-cadherin. (F) Western blot analysis of E-cadherin protein expression in MDA-MB-231 or MCF-7 cells after transfection with LINC00324 overexpression vectors or LINC00342 siRNA. (G) Transwell assay indicating migration of MDA-MB-231 cells after transfection with LINC00324 overexpression vectors and miR-10b-5p mimics. (H) Immunofluorescence staining of E-cadherin (Green) in indicated MCF-7 cells. Scale bar = 50 μm. (I, J) qRT-PCR analysis of CDH1 mRNA level (I) or western blot analysis of E-cadherin protein level (J) in indicated MCF-7 cells. All data are shown as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are from three independent experiments (C, I), or are representative of three independent experiments with similar results (D, FH, J).