Research Paper Volume 12, Issue 7 pp 6340—6351

Leptin acts on mesenchymal stem cells to promote chemoresistance in osteosarcoma cells

Figure 1. CM from leptin-conditioned MSCs promotes chemoresistance in OS cells. Results of CCK8 viability assays in cisplatin-treated MG-63 cells (A) and U-2 OS cells (B) incubated with CM collected from MSCs cultured in the presence (CM) or absence (Control) of leptin. *p<0.05; **p<0.01. Apoptosis was detected by PI/Annexin V-FITC flow cytometry in cisplatin-treated MG-63 cells (C) and U-2 OS cells (D) incubated with CM collected from MSCs cultured in the presence (CM) or absence (Control) of leptin. *p<0.05; **p<0.01. (E) Representative images of human OS xenografts excised from nude mice. (F) Quantification of tumor volumes at 21 days post-implantation. *p<0.05; ***p<0.001.