Figure 3. FAM46C knockdown promoted cell growth of 22RV1 and DU145 cells. (A, B) The efficiency of three pLKO.1-shRNAs in silencing endogenous FAM46C in 22RV1 and DU145 cells was measured by qPCR and western blot. After 22RV1 and DU145 cells were transduced with pLKO.1-shFAM46C#1 and pLKO.1-shFAM46C#3, the cell proliferation (C–E), cell cycle (F) and apoptosis (G) were measured by CCK-8, colony formation and flow cytometry, respectively. ***P<0.001 compared with pLKO.1-shNC.