Research Paper Volume 12, Issue 7 pp 6352—6369

FAM46C inhibits cell proliferation and cell cycle progression and promotes apoptosis through PTEN/AKT signaling pathway and is associated with chemosensitivity in prostate cancer

Figure 8. Higher FAM46C expression enhanced the chemosensitivity of prostate cancer cells. (A) CCK-8 showing the effect of pLVX-Puro-FAM46C lentivirus transduction on the proliferation of DU145cells treated with 10 nM docetaxel. (B) CCK-8 showing the effect of pLKO.1-shFAM46C transduction on the proliferation of 22RV1 cells treated with 10 nM docetaxel. ***P<0.001 compares with vector+vehicle; ###P<0.001 compared with FAM46C+vehicle. (C, D) Tumor weight and volume were measured in mice with PDX model following 10 mg/kg docetaxel chemotherapy (n=4). (E) FAM46C mRNA expression levels were measured by qPCR in the tumor cells isolated from primary prostate cancer patients from hospital cohort (n=12). (F) After primary isolated prostate cancer cells (n=12) were treated with 10 nM docetaxel, the inhibition rates of cell proliferation was measured (24 h relative to 0 h). *P<0.05, **P<0.01, ***P<0.001.