Research Paper Volume 12, Issue 7 pp 6385—6400

Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosis

Figure 4. Effects of NORAD-knockdown on ox-LDL-induced proinflammatory molecules in HUVECs. (A) mRNA expression of ICAM-1 in ox-LDL-treated HUVECs observed through qRT-PCR (n = 3, *P < 0.05 vs. siCTRL, ##P < 0.01 vs. siCTRL+ox-LDL). (B) mRNA expression of VCAM in ox-LDL-treated HUVECs observed through qRT-PCR (n = 3, **P < 0.01 vs. siCTRL, #P < 0.05 vs. siCTRL+ox-LDL). (C) mRNA expression of IL-8 mRNA in ox-LDL-treated HUVECs observed through qRT-PCR (n = 3, **P < 0.01 vs. siCTRL, #P < 0.05 vs. siCTRL+ox-LDL). (D) RIP assay confirmed the interaction of NORAD and SFPQ. HUVEC lysates were incubated with the anti-SFPQ or anti-IgG antibody, and the precipitated complexes were analyzed with qRT-PCR to investigate the expression of NORAD (n = 3, *P < 0.05 vs. IgG).