Figure 2. Docetaxel resistance in HSC-3 cells (HSC-3DR) was associated with EMT and elevated drug efflux. (A) Migration ability of HSC-3 and HSC-3DR cells was determined by wound healing assays (scale bars = 100 μm). (B) The expressions of EMT-associated proteins in HSC-3DR cells were determined by western blots. (C) The expression of nuclear γ-H2AX of HSC-3 and HSC-3DR cells was determined by fluorescence assays (scale bars = 10 μm). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.