Research Paper Volume 12, Issue 8 pp 6756—6773

Exosomal miR-200c suppresses chemoresistance of docetaxel in tongue squamous cell carcinoma by suppressing TUBB3 and PPP2R1B

Figure 3. Downregulation of miR-200c was involved in docetaxel resistance in HSC-3 cells (HSC-3DR). (A) volcano plot of RNA-Seq analysis. Red and green points represent significantly upregulated and downregulated miRNAs, respectively, according to fold change > 2 and adjusted p < 0.05. (B) Expressions of downregulated miRNAs in RNA-Seq analysis were verified by qRT-PCR. (C) The expression of miR-200c in tongue squamous cell carcinoma cell lines was determined by qRT-PCR. (D) The expression of miR-200c in HSC-3 and HSC-3DR cells was determined by qRT-PCR. (E) The efficiency of miR-200c-encoding lentiviral vectors in HSC-3DR cells was determined by qRT-PCR. (F) Cell viability in HSC-3DR cells treated with miR-200c-encoding lentiviral vector (LV-200c) was determined by CCK8 assays. (G) Apoptosis of HSC-3DR cells treated with miR-200c-encoding lentiviral vectors (LV-200c) was determined by flow cytometry. (H) Invasion ability of HSC-3DR cells treated with miR-200c-encoding lentiviral vector (LV-200c) was determined by Transwell assays (scale bars = 50 μm). (I) Migration ability of HSC-3DR cells treated with miR-200c-encoding lentiviral vector (LV-200c) was determined by Transwell assays (scale bars = 50 μm). Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.