Research Paper Volume 12, Issue 8 pp 6756—6773

Exosomal miR-200c suppresses chemoresistance of docetaxel in tongue squamous cell carcinoma by suppressing TUBB3 and PPP2R1B

Figure 4. Effect of miR-200c on docetaxel resistance in in vitro and in vivo. (A) Migration ability of HSC-3DR cells treated with miR-200c-encoding lentiviral vectors (LV-200c) was determined by wound healing assays (scale bars = 100 μm). (B) The expressions of EMT-associated proteins in HSC-3DR cells treated with miR-200c-encoding lentiviral vectors (LV-200c) were determined by western blots. (C) The expression of nuclear γ-H2AX in HSC-3DR cells treated with miR-200c-encoding lentiviral vectors (LV-200c) was determined by fluorescence assays (scale bars = 10 μm). (D) Tumor volume of xenograft mice treated with HSC-3DR cells treated with miR-200c-encoding lentiviral vectors (LV-200c) and DTX. (E) Representative images of tumors. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.