Research Paper Volume 12, Issue 8 pp 6756—6773

Exosomal miR-200c suppresses chemoresistance of docetaxel in tongue squamous cell carcinoma by suppressing TUBB3 and PPP2R1B

Figure 5. Characteristics and effects of exosomes derived from normal tongue epithelial cells (NTEC) transfected with miR-200c. (A) The morphology and size distribution of exosomes were determined by electron microscopy and nanoparticle tracking analysis (left figure: scale bars = 200 μm and middle figure: scale bars = 100 μm). (B) The expressions of exosome markers were determined by western blots. (C) The internalization of exosomes was determined by fluorescence assays. Blue: nuclei labeled with DAPI. Green: miR-200c-carrying exosomes labeled with PKH67. (D) The expression of miR-200c was determined by qRT-PCR in NTEC transfected with miR-200c-encoding lentiviral vectors (LV-200c) and their exosomes. (E) The expression of miR-200c was determined by qRT-PCR in exosomes treated with RNase A or the combination of RNase A and Triton X-100. (F) The expression of miR-200c was determined by qRT-PCR in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor. (G) Cell viability was determined by CCK8 assays in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.