Research Paper Volume 12, Issue 8 pp 6756—6773

Exosomal miR-200c suppresses chemoresistance of docetaxel in tongue squamous cell carcinoma by suppressing TUBB3 and PPP2R1B

Figure 7. MiR-200c-carrying exosomes regulated docetaxel resistance in in vitro and in vivo. (A) Migration ability was determined by wound healing assays in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor (scale bars = 100 μm). (B) The expression of nuclear γ-H2AX was determined by fluorescence assays in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor (scale bars = 10 μm). (C) The expressions of EMT-associated proteins were determined by western blots in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor. (D) Tumor volume of xenograft mice treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor. (E) Representative images of tumors. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.