Research Paper Volume 12, Issue 8 pp 6756—6773

Exosomal miR-200c suppresses chemoresistance of docetaxel in tongue squamous cell carcinoma by suppressing TUBB3 and PPP2R1B

Figure 9. MiR-200c-carrying exosomes regulated expressions of TUBB3 and PPP2R1B. (A) Migration ability was determined by wound healing assays in HSC-3DR cells transfected with TUBB3 or PPP2R1B-carrying vectors with the wild-type or mutant miR-200c sequence (scale bars = 100 μm). (B) The expression of nuclear γ-H2AX was determined by fluorescence assays in HSC-3DR cells transfected with TUBB3 or PPP2R1B-carrying vectors with the wild-type or mutant miR-200c sequence (scale bars = 10 μm). (C) The protein expressions of TUBB3 and PPP2R1B were determined by western blots in HSC-3DR cells treated with miR-200c-carrying exosomes or miR-200c-carrying exosomes with the miR-200c inhibitor. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.