Research Paper Volume 12, Issue 8 pp 6774—6792

circTNFRSF21, a newly identified circular RNA promotes endometrial carcinoma pathogenesis through regulating miR-1227-MAPK13/ATF2 axis

Figure 3. circTNFRSF21 promotes EC cell growth, proliferation and in vivo tumor formation. (A) circTNFRSF21 expression in EC patient tumor tissues and adjacent normal tissues. (B) circTNFRSF21 expression in EC cell lines and HEuEC cells. Cell growth (C) and cell cycle (D) detection after overexpressing circTNFRSF21 in HEuEC cells. (E) Cell growth after silencing circTNFRSF21 in HEC1-A and Ishikawa cells. (F) Cell colony formation after silencing circTNFRSF21 in HEC1-A and Ishikawa cells. Statistical results of colony number were shown on the right. (G) Cell cycle and (H, I) Cell apoptosis after silencing circTNFRSF21 in HEC1-A and Ishikawa cells. Etoposide induced cell apoptosis was used as positive control. (J) Image of tumors derived from si-circTNFRSF21 and control Ishikawa cells in nude mice (n=6). (K) Tumor weights and (L) growth curve of tumors from the 2 groups. *P<0.05, **P<0.01, ***P<0.001. Data are represented as mean +/- SEM.