Figure 5. circTNFRSF21 acts as a sponge of miR-1227 in vivo. (A) Diagram of circ_TNFRSF21 and miR-1227 interactions (B) Pull down miR-1227 with biotin labeled WT circTNFRSF21 or miR-1227 binding sites mutated circ_TNFRSF21 from HEC1-A cell lysis. Detection of miR-1227 expression after overexpressing (C) or knocking down (D) circTNFRSF21 in HEC1-A and Ishikawa cells. (E) qRT-PCR detect miR-1227 expression in EC cell lines and HEuEC. (F) miR-1227 expression in in EC patient tumor tissues and adjacent normal tissues. (G) Cell growth after overexpressing miR-1227 in HEC1-A and Ishikawa cells. (H) Cell growth after silencing miR-1227 in HEC1-A and Ishikawa cells. (I, J) Cell apoptosis detection after silencing miR-1227 in HEC1-A and Ishikawa cells. *P<0.05, **P<0.01, ***P<0.001. Data are represented as mean +/- SEM.