Research Paper Volume 12, Issue 8 pp 6774—6792

circTNFRSF21, a newly identified circular RNA promotes endometrial carcinoma pathogenesis through regulating miR-1227-MAPK13/ATF2 axis

Figure 7. circTNFRSF21 rescues MAPK13-ATF2 signaling pathway by acting as miR-1227 sponge in EC cells. Detection of MAPK13 transcription (A, B) and protein level (C). (D, E) Luciferase assay detect MAPK13 3’UTR activity. (F, G) Cell growth detection after knocking down MAPK13 in the presence or absence or circTNFRSF21. (H) Western blot detects MAPK13, ATF2, phosphorylated ATF2 expression. GAPDH was used as internal control. (I) Cell growth detection after knocking down MAPK13 in the presence or absence or ATF2. (J) Western blot detects MAPK13, ATF2 expression, GAPDH was used as internal control. *P<0.05, **P<0.01, ***P<0.001. Data are represented as mean +/- SEM.