Research Paper Volume 12, Issue 7 pp 6415—6435

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

Figure 2. ES inhibits VbP-induced pyroptosis in THP-1 macrophages. (A) Pattern diagram of the ES device. (B) Circuit diagram of the ES device. (C) Viability assay results in control THP-1 macrophages after ES at (1) different voltages (9 min exposure) and (2) 30 mv/cm for various exposure times. (D) Viability assay results in macrophages exposed to VbP (15 μM, 72 h) and subsequently treated with ES at (1) different voltages (9 min exposure) and (2) 20 mv/cm for different exposure times. (E) Viability measurements at different time points after ES (20 mv/cm for 9 min) in macrophages treated with VbP (15 μM, 72 h). (F) Western blot analysis of NLRP3 and caspase-1 protein expression at different time points after ES. (G) Dose-response analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (9 min exposure) after VbP treatment (15 μM, 72 h). (H) Time-dependent analysis of the expression of pyroptosis-related proteins in macrophages treated with ES (20 mv/cm) after VbP exposure (15 μM, 72 h). n =3; *P<0.05, **P<0.01, and ***P<0.001 vs. control cells.