Research Paper Volume 12, Issue 7 pp 6415—6435

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

Figure 3. ES inhibits caspase-1-dependent pyroptosis of THP-1 macrophage induced by vbp. (A) Western blot analysis of the effect of caspase-1 inhibition on pyroptosis-related proteins. (B) RT-qPCR analysis of the effect of caspase-1 inhibition on pyroptosis-related mRNAs. (C) Representative western blot results showing downregulation of caspase-1 expression following siRNA-mediated caspase-1 knockdown. (D) Western blot analysis of the effect of caspase-1 knockdown on pyroptosis-related proteins. n = 3; *P<0.05, **P<0.01, and ***P<0.001 vs. control cells; #P<0.05, ##P<0.01, and ###P<0.001 vs. VbP-treated cells.