Research Paper Volume 12, Issue 7 pp 6436—6455

Sevoflurane inhibits neuronal migration and axon growth in the developing mouse cerebral cortex

Figure 5. Nova2 regulates neuronal migration through modulating Netrin-1/Dcc activity in the developing mouse cerebral cortex. (A) Dual sevoflurane exposure significantly decreased neuronal migration cortex of offspring mice, and Nova2 overexpression can rescue the neuronal migration deficits induced by dual sevoflurane exposure. While Netrin-1/Dcc shRNA mitigated the Nova2 rescue phenomenon, which was similar to the neuronal migration deficits induced by dual sevoflurane exposure. (B) Quantification of GFP+ cells at different positions in different groups. Similar with dual sevoflurane exposure group, Netrin-1/Dcc knockdown mitigated the Nova2 rescue phenomenon, larger fractions of neurons in the IZ (F = 5.672, P = 0.0634, N = 3, one-way ANOVA) and the VZ/SVZ (F =14.68, P = 0.0126*, N = 3, one-way ANOVA), and a significantly smaller fraction of neurons in the UpCP (F = 35.79, P = 0.0024**, one-way ANOVA). *P<0.05. **P<0.01, ***P<0.001.****P < 0.0001.