Figure 1. Modulation of FGF22 and MEF2D levels in the cultured mouse hair cells. (A) Isolated mouse hair cells in culture and immunostained with Myosin7a (in red). (B, C) Cultured hair cells were transduced with AAV-FGF22 or AAV-shFGF22 or control AAV-scr (scramble) and assessed for FGF22 levels by RT-qPCR (B), and by ELISA (C). (D, E) Cultured hair cells were transduced with AAV-MEF2D or AAV-shMEF2D or control AAV-scr (scramble) and assessed for MEF2D levels by RT-qPCR (D), and by ELISA (E). *p<0.05. N=5. Scale bar is 50μm.