Research Paper Volume 12, Issue 7 pp 6456—6466

FGF22 promotes generation of ribbon synapses through downregulating MEF2D

Figure 4. FGF22-induced calcium influx activates CalN to inhibit MEF2D in mouse hair cells. (A, B) CalN levels in transduced hair cells by RT-qPCR (A), and by ELISA (B). (C) NFATc levels in transduced hair cells by RT-qPCR. (D, E) Cultured hair cells were transduced with AAV-CalN or AAV-shCalN or control AAV-scr (scramble) and assessed for MEF2D levels by RT-qPCR (D), and by ELISA (E). *p<0.05. NS: non-significant. N=5.