Research Paper Volume 12, Issue 8 pp 6808—6822

FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway

Figure 4. Effect of FAM84B on tumor progression via regulation of the β-catenin pathway. (A) GSEA analysis revealed that FAM84B expression was positively correlated with CTNNB1 oncogenic signature in TCGA PDAC dataset. NES: normalized enrichment score. (B) Western blotting analysis of nuclear β-catenin, and protein expression of c-Myc, LDHA and Survivin in AsPC-1 and CFPAC1 cells with FAM84B knockdown. (C) PANC-1 cells were transduced with lentivirus expressing Vector/FAM84B for 48 h and western blotting analysis was performed to assess FAM84B expression. (DJ) PANC-1 cells were transduced with lentivirus expressing Vector/FAM84B, and then treated with vehicle (DMSO, vehicle) or 10 μM XAV939. Cell growth (D), apoptosis (E), extracellular acidification rates (ECAR, F), oxygen consumption (OCR, G), 2-NBDG uptake (H) and lactate production (I), as well as nuclear β-catenin and the protein levels of c-Myc, LDHA and Survivin (J) were detected. **P < 0.01 and ***P<0.001.