Research Paper Volume 12, Issue 8 pp 6865—6879

Circular RNA circRGNEF promotes bladder cancer progression via miR-548/KIF2C axis regulation

Figure 7. KIF2C overexpression reversed miR-548-induced cell migration, invasion, and growth inhibition in vitro. (AD) T24 and UM-UC-3 cells were transfected with miR-548 mimics with or without the KIF2C overexpression vector. RT-qPCR shows the expression of miR-548 (A, B) and KIF2C (C, D) in T24 and UM-UC-3 cells. Data are denoted by the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. mimic. (EH) EdU assay showing the proliferation of T24 (E, F) and UM-UC-3 (G, H) cells. Data are denoted by the mean ± SD. *P < 0.05, ***P < 0.001. ###P < 0.001 vs. mimic. (IM) Cell migration and invasion were determined in T24 and UM-UC-3 cells by Transwell assays. Data are denoted by the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. mimic.