Research Paper Volume 12, Issue 8 pp 6880—6890

PP2A subunit PPP2R2C is downregulated in the brains of Alzheimer’s transgenic mice

Figure 2. PPP2R2C are differentially expressed in mouse brain tissues throughout lifespan. (A) Quantitative RT-qPCRs for PPP2R2C were analyzed in wildtype mouse cortex, cerebellum, heart, liver, muscle, lung, skin and ovary at 2 time-points respectively (3 month and 12 month, n=9 each). Significance was tested between cortex, cerebellum, heart, lung, skin and ovary. Each measure represents the average fold-change expression of nine independent repetitions (Biological triplicate in technical RT duplicate) normalized to two housekeeping genes (β-actin and 36B4); ΔΔCt method). Mean+/-SEM with associated statistical significance are reported (*p<0.05, **p<0.01). (B) Representative image and the quantification from immunoblots of PPP2R2C antibody in wildtype (Wt) and transgenic (Tg) mouse cortex at different time-points of lifespan. Data are shown in mean+/-SEM, n=9 each condition, *p<0.05, **p<0.01. (C) Quantitative RT-qPCRs for PPP2R2C in wildtype (Wt) and transgenic (Tg) mouse cortex at different time-points of lifespan from 3 month to 18 months. Each measure represents the average fold-change expression of nine independent repetitions (Biological triplicate in technical RT duplicate) normalized to two housekeeping genes (β-actin and 36B4); ΔΔCt method). Mean+/-SEM with associated statistical significance are reported between 9, 12 and 16 month (*p<0.05, ***p<0.001).