Research Paper Volume 12, Issue 8 pp 6880—6890

PP2A subunit PPP2R2C is downregulated in the brains of Alzheimer’s transgenic mice

Figure 3. Detection of PPP2R2C expression in different neural cells isolated from mice brains. (A) Representative images of confocal sections from cultured primary cells (neural stem cell, NSC; astrocyte, Glia and neuron) isolated from wildtype newborn mouse brain, staining with of PPP2R2C antibody (red) and neural markers (green) and counterstained with DAPI (blue). (B) Quantification of the mean number of PPP2R2C foci per nucleus and Cytoplasm of (A) normalized to the number of nuclei. Data are presented at mean+/-SEM. (C) Quantitative RT-qPCRs analyzing PPP2R2C RNA level (n=3) and (D) Representative images of confocal sections staining with of PPP2R2C antibody (red) and neural markers (green) and couterstained with DAPI (blue) in glia cells isolated from wildtype newborn mouse brain after silencing of PPP2R2C. Scale bar 5 um, * p<0.05.