Research Paper Volume 12, Issue 8 pp 6904—6927

Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6

Figure 3. The exploration and verification of downstream underlying UAP1L1 induced regulation of gastric cancer. (A) A PrimeView Human Gene Expression Array was performed to identify the differentially expressed genes (DEGs) between shUAP1L1 and shCtrl groups of SGC-7901 cells. (B) Western blotting was used to detect the expression of several selected DEGs in SGC-7901 cells with or without UAP1L1. (C) A UAP1L1 associated interaction network constructed by IPA analysis revealed the potential linkage between UAP1L1 and CDK6. (D) The expression of CDK6 in gastric cancer tissues and normal tissues was evaluated by IHC analysis (scale bar = 50 μm). (E) Data mining of expression profiling in TCGA database showed the upregulated expression of CDK6 in gastric cancer tissues compared with normal tissues. (F) Data mining of prognosis in KM plotter database showed that patients with higher expression of CDK6 suffered from shorter survival period. (G) The direct interaction between UAP1L1 and CDK6 was demonstrated by co-immunoprecipitation. (H) Data mining of expression profiling in TCGA database revealed the positive correlation between UAP1L1 and CDK6 in gastric cancer tissues. The representative images were selected from at least 3 independent experiments. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.