Research Paper Volume 12, Issue 8 pp 6928—6946

AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling

Figure 6. Loss of AKT3 delayed cutaneous wound healing in mice. (A) Schematic of AKT3 knockout in mice. (B) Western blotting for AKT3 levels in AKT3+/+ and AKT3-/- mice (n = 6). (C) AKT3 knockout delayed cutaneous wound healing in mice by days 7 and 14 post-injury. (Dac) Histological staining of mouse cutaneous wound tissue. (a) H&E staining showed more inflammatory cells in the wound tissue of AKT3-/- mice and incomplete tissue integrity (n = 6). (b) Masson staining showed the numbers of collagenous and muscular fibers were reduced in the wound tissue of AKT3-/- mice (n = 6). (c) EVG staining showed that number of elastin fibers were decreased in the wound tissue of AKT3-/- mice (n = 6). (E) IF staining showed the F40/80 and CD206 expression in mouse cutaneous wound tissue. All the experiments were repeated at least three times.