Research Paper Volume 12, Issue 8 pp 6947—6965

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway

Figure 2. Differentiation potential of the ADSCs in the high glucose environment. (A) Adipogenic potential differentiation of ADSCs by oil-red staining; (B) Osteogenic differentiation potential evaluation of ADSCs by alizarin-red staining; (C) flow cytometry analysis for oxidative stress of hADSCs from different sources; (D) (E) The angiogenesis potential of the cells was detected and the tube length of the cells were measured; (F) The mRNA expression of the angiogenesis-genes in different hADSCs by RT-qPCR analysis; (G)(H) The protein expression of the angiogenesis-genes in different hADSCs by western blot analysis (* P<0.05; Scale bar = 100 μm).