Research Paper Volume 12, Issue 8 pp 6947—6965

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway

Figure 5. Regulatory role of miR-1248 in stem cell ability of hADSCs. (A) The expression of miR-1248 in miR-1248 inhibitor or mimics in different hADSCs by RT-qPCR; (B) MiR-1248 regulated proliferative capacity of hADSCs against glucolipotoxicity; (C) MiR-1248 regulated stem cell invasion and migration capacity against glucolipotoxicity; (D) Flow cytometry for oxidative stress of hADSC against glucolipotoxicity; (E) The protein expression of angiogenesis-related gene in hADSC affected by miR-1248 was detected by western blot; (F) The mRNA expression of angiogenesis-related gene in hADSC affected by miR-1248 was detected by RT-qPCR; (G) The angiogenesis potential of the cells was detected and the tube length of the cells were measured (* P<0.05).