Figure 6. SIRT1 interacts with CREB. (A) GST-CREB or GST coupled onto glutathione sepharose was incubated with the extract of HEK-293A cells overexpressing SIRT1. After washing, bound proteins were subjected to western blots using anti-GST and anti-SIRT1 antibody. (B) SIRT1 could be co-immunoprecipitated by HA-CREB using anti-HA antibody. CREB tagged with HA and SIRT1 tagged with Myc were co-expressed in HEK-293A cells for 48 h. The cell extract was incubated with anti-HA antibody coupled onto protein G beads. The bound proteins were subjected to western blots using anti-HA antibody to CREB and anti-Myc antibody to SIRT1.