Research Paper Volume 12, Issue 8 pp 7066—7111

Cutaneous wound healing in aged, high fat diet-induced obese female or male C57BL/6 mice

Figure 7. Collagen expression, hydroxyproline content and fibrosis localization in uninjured and post-injured skin.Collagen 1 (A) and collagen 3 (B) mRNA expression (n=4-8 skin samples per group) in skin tissues of old, young, male (M), female (F), LFD or HFD mice. Hydroxyproline (C) content (n=4-8 skin samples per group) in skin tissues of old, young, LFD or HFD mice. Histological skin sections stained with Masson trichrome collected from young or old mice fed LFD or HFD at post-injured day 14 and 21 (D), followed by quantification of the fibrosis area (E) (n=3 mice per group). Scale bar (E) 200 μm. The bars indicate lsmean ±SE *p<0.05, **p<0.01, ***p<0.001.