Figure 4.XIST regulates the expressions of miR-101-3p and FOS in NMCM under anoxia condition. (A) The expression of XIST was measured by qRT-PCR (n=3). NMCMs were transfected with miR-101a-3p inhibitor or mimic. (B) The expression of miR-101a-3p was measured by qRT-PCR. Knockdown of XIST increases the expression levels of miR-101a-3p under anoxia. NMCMS were infected with XIST-siRNA or XIST-sc (n=3). (C, D) The expression of FOS and c-FOS was measured by qRT-PCR and western blot. Knockdown of XIST decreases the expression levels of FOS and c-FOS under anoxia. NMCMS were infected with XIST-siRNA or XIST-sc (n=3). Cells were transfected with miR-101a-3p mimic, inhibitor, S3 or si-RNA control (Sc) and placed in hypoxia chamber for 8 h to induce anoxia condition. Data are shown as mean ± SD, * P<0.05.
