Research Paper Volume 12, Issue 8 pp 7248—7261

MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B

Figure 2. MiR-375 targeting ATG2B.3’-UTR. (A) miR-375 targeting the 3’-UTR of ATG2B predicted by TargetScan; (B) miR-375 mimics significantly reduced the wild 3’-UTR luciferase reporter activity of ATG2B; (C) OA chondrocytes were transfected with miRNA-375 mimics or inhibitor, and miRNA-375 expression was detected by qRT-PCR; (D, E) OA chondrocytes were transfected with pcDNA3.1-ATG2B, and ATG2B protein was detected by western blot, and the statistical results were presented; (F, G) protein expression of ATG2B, Collagen II and MMP13 were measured by western blot, and the statistical results were presented. * P<0.05, ** P<0.01, compared with NC group; #, P<0.05, compared with pcDNA3.1-ATG2B group.