Research Paper Volume 12, Issue 8 pp 7313—7333

Estrogen regulation of germline stem cell differentiation as a mechanism contributing to female reproductive aging

Figure 5. Targeted disruption of the Esr1 gene impairs E2-driven oogenesis during adulthood. (A) Ovarian Stra8 mRNA levels in young adult Esr1–/– and Esr2–/– mice (versus respective WT littermates) 24 hours after injection of vehicle (V) or E2 (0.5 mg/kg) (mean ± SEM, n = 5–6 mice per group; *P<0.05). (B) Primordial follicle numbers in ovaries of young adult Esr1–/– and Esr2–/– mice (versus respective WT littermates) 24 hours after injection of V or E2 (0.5 mg/kg) (mean ± SEM, n = 4–6 mice per group; *P<0.05). (C, D) Numbers of primordial follicles (C) and Stra8 mRNA levels (D) in ovaries of Esr1–/– and Esr2–/– mice (versus respective WT littermates) at 2 months of age (mean ± SEM, n = 4–6 mice per group; *P<0.05).