Research Paper Volume 12, Issue 8 pp 7491—7510

RNA-seq analysis of potential lncRNAs for age-related hearing loss in a mouse model

Figure 7. Correlation of lncRNA NONMMUT010961.2 and its potential target mRNAs. (A) The chromosome location of NONMMUT010961.2 in the mouse genome is shown. (B, C) Pearson’s correlation was used to analyze the positive correlation between lncRNA NONMMUT010961.2 expression and the expression level of Hgf and Ar in the 12 matched samples. (D) The relative expression level of lncRNA NONMMUT010961.2 in HEI-OC1 cells treated with lncRNAi for 24 h, 36 h, 48 h and 72 h compared with those treated with NC. *P < 0.05 compared to NC. (E) qRT-PCR analysis showed that lncRNA NONMMUT010961.2 and its potential target mRNAs Hgf and Ar decreased in HEI-OC1 cells treated with lncRNAi for 36 h compared with those treated with NC. *P < 0.05 compared to NC. (F) Western blot analysis showed that cleaved caspase 3 and HO-1 were decreased by lncRNAi in the AHL cellular model. (*P < 0.05).